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phosphorylated smad1/5 (ser463/465) clone 41d10  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc phosphorylated smad1/5 (ser463/465) clone 41d10
Phosphorylated Smad1/5 (Ser463/465) Clone 41d10, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. TMP269 increases BMP2 expression and promotes <t>BMP-Smad-dependent</t> transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.
Phosphorylated Smad Psmad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated smad psmad/product/Cell Signaling Technology Inc
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anti- phosphorylated smad1/5 antibody  (Cell Signaling Technology Inc)
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Fig. 4. TMP269 increases BMP2 expression and promotes <t>BMP-Smad-dependent</t> transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.
Anti Phosphorylated Smad1/5 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti- phosphorylated smad1/5 antibody/product/Cell Signaling Technology Inc
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phosphorylated smad 1 5  (Cell Signaling Technology Inc)
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Fig. 4. TMP269 increases BMP2 expression and promotes <t>BMP-Smad-dependent</t> transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.
Phosphorylated Smad 1 5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. TMP269 increases BMP2 expression and promotes <t>BMP-Smad-dependent</t> transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.
Phosphorylated Smad1 5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated smad1 5/product/Cell Signaling Technology Inc
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receptor  (Cell Signaling Technology Inc)
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Fig. 4. TMP269 increases BMP2 expression and promotes <t>BMP-Smad-dependent</t> transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.
Receptor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/receptor/product/Cell Signaling Technology Inc
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phosphorylated smad1/5/8 antibody  (Cell Signaling Technology Inc)
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Fig. 4. TMP269 increases BMP2 expression and promotes <t>BMP-Smad-dependent</t> transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.
Phosphorylated Smad1/5/8 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated smad1/5/8 antibody/product/Cell Signaling Technology Inc
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Fig. 4. TMP269 increases BMP2 expression and promotes BMP-Smad-dependent transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.

Journal: Neuropharmacology

Article Title: The class-IIa HDAC inhibitor TMP269 promotes BMP-Smad signalling and is neuroprotective in in vitro and in vivo 6-hydroxydopamine models of Parkinson's disease.

doi: 10.1016/j.neuropharm.2025.110319

Figure Lengend Snippet: Fig. 4. TMP269 increases BMP2 expression and promotes BMP-Smad-dependent transcription in SH-SY5Y cells. (A) Schema showing the BMP-Smad pathway and illustrating that HDAC5 binds to the BMP2 promoter. (B–F) Graphs showing RT-qPCR data for (B) BMP2, (C) BMPR2, (D) BMPR1B, (E) SMAD1 and (F) SMAD5 mRNA levels relative to the levels of the geometric mean of three reference mRNAs, GAPDH, TBP and B2M, in SH-SY5Y cells treated for 24 h with 0.1 μM TMP269. (G) Schema showing the BRE-GFP reporter and representative photomicrographs of BRE-GFP-transfected SH-SY5Y cells, with and without TMP269 treatment. Scale bar = 50 μm. (H) Graph showing densitometric quantification of GFP fluorescence intensity in SH-SY5Y cells, with and without TMP269 treatment. All data are presented as the mean ± SEM from n = 3 or 4 independent experiments. *p < 0.05, **p < 0.01; Student’s t-test.

Article Snippet: The primary antibodies were: tyrosine hydroxylase (TH) (Millipore MAB318; 1:200), BMP2 (Abcam; 1:500) acetylated histone H3 (Santa Cruz Biotechnology sc-56616; 1:200) phosphorylated-Smad (pSmad) 1/5/9 (1:500; Cell Signalling #9516S).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Fluorescence

Fig. 5. TMP269 increases BMP2 and pSmad1/5 expression and protects against 6-OHDA-induced decreases in neurite growth through the BMP-Smad pathway in SH- SY5Y cells. Representative photomicrographs of SH-SY5Y cells treated with 5 μM 6-OHDA and cultured with or without 0.1 μM TMP269 for 72h before immuno staining for (A) BMP2 or (C) pSmad1/5. Densitometric quantification of (B) BMP2 expression and (D) pSmad1/5 in SH-SY5Y cells treated with 5 μM 6-OHDA and cultured with or without 0.1 μM TMP269 for 72h. (E) Representative photomicrographs and (F) neurite length analysis of SH-SY5Y cells treated with 5 μM 6-OHDA and cultured with or without 0.1 μM TMP269 and with or without 1 μg/ml dorsomorphin for 72h. Scale bar = 50 μm. All data are presented as the mean ± SEM from n = 3–4 independent experiments. *p < 0.05, **p < 0.01, ****p < 0.0001; Two-way ANOVA with post-hoc Tukey’s test.

Journal: Neuropharmacology

Article Title: The class-IIa HDAC inhibitor TMP269 promotes BMP-Smad signalling and is neuroprotective in in vitro and in vivo 6-hydroxydopamine models of Parkinson's disease.

doi: 10.1016/j.neuropharm.2025.110319

Figure Lengend Snippet: Fig. 5. TMP269 increases BMP2 and pSmad1/5 expression and protects against 6-OHDA-induced decreases in neurite growth through the BMP-Smad pathway in SH- SY5Y cells. Representative photomicrographs of SH-SY5Y cells treated with 5 μM 6-OHDA and cultured with or without 0.1 μM TMP269 for 72h before immuno staining for (A) BMP2 or (C) pSmad1/5. Densitometric quantification of (B) BMP2 expression and (D) pSmad1/5 in SH-SY5Y cells treated with 5 μM 6-OHDA and cultured with or without 0.1 μM TMP269 for 72h. (E) Representative photomicrographs and (F) neurite length analysis of SH-SY5Y cells treated with 5 μM 6-OHDA and cultured with or without 0.1 μM TMP269 and with or without 1 μg/ml dorsomorphin for 72h. Scale bar = 50 μm. All data are presented as the mean ± SEM from n = 3–4 independent experiments. *p < 0.05, **p < 0.01, ****p < 0.0001; Two-way ANOVA with post-hoc Tukey’s test.

Article Snippet: The primary antibodies were: tyrosine hydroxylase (TH) (Millipore MAB318; 1:200), BMP2 (Abcam; 1:500) acetylated histone H3 (Santa Cruz Biotechnology sc-56616; 1:200) phosphorylated-Smad (pSmad) 1/5/9 (1:500; Cell Signalling #9516S).

Techniques: Expressing, Cell Culture, Immunostaining